Fruvedomics is a WVCTSI-funded pilot research study focused on the identification of biomarkers for poor metabolic, cardiovascular, and microbiome health, as well as their sensitivity to nutritional intervention in young adults.
Introduction
Chronic diseases are a major public health concern nationwide, but particularly in the health disparate regions often seen in rural states, such as West Virginia. It is becoming increasingly critical that we identify the distinct role of nutrition in the etiology of chronic diseases and understand how specific diets influence the metabolome. Furthermore, isolating specific biomarkers and determining their effects on the microbiome will be necessary to the development of individualized therapeutic diet plans that effectively prevent chronic diseases both at the individual level and on a much larger community scale.
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Aims
Fruvedomics uses a translational approach to a free-living, nutrition intervention based on the 2010 Dietary Guidelines for Americans. This study aims to:
- investigate dietary impact on the metabolome, microbiome, and cardiovascular system.
- identify novel metabolic phenotypes.
- assess behavior change outcomes in college-aged students at-risk of metabolic syndrome.
Design
Fruvedomics
is a translational clinical research trial. A total of 36 subjects, most of whom
screened as “at risk” for metabolic syndrome, participated in an 8-week nutritional
intervention study. Subjects were randomized into one of three groups:
- “Fruved” diet with 50% fiber, fruit, and vegetable
- “Fruved” and low simple-carbohydrate diet (Fruved+LowCHO)
- “Fruved” and low-fat diet (Fruved+LowFat).
As part of our metabolomics approach, venous blood samples were collected for amino acid, carbohydrate, fatty acid, and sphingolipid metabolism. Collections occurred pre- and post-nutritional intervention, and at weeks three and five of the program, for a total of four repeated blood samples for metabolomic assessment. Additionally, anthropometrics, body composition, stool samples, and arterial stiffness measurements were recorded pre- and post-intervention. Finally, participants answered a ~300-question lifestyle behaviors survey pre- and post-intervention.
Diet were measured both subjectively (food pictures/diet logs) and objectively (nutritionist
professional/food receipts) on a weekly basis throughout the eight-week intervention
period. Each week the subject was incentivized to have their logs, receipts, and
pictures reviewed by a Registered Dietitian Nutritionist in order to facilitate
counseling and ensure compliance with the diet.
Check out some post intervention results.
Outcomes, Impact, & Future Work
Preliminary findings of "at-risk" students show improved metabolic health after a diet intervention of increased fruits and vegetables. Changes in the metabolome and microbiome were found, with results indicating less inflammation, which is associated with lower disease risk. Individuals with metabolic syndrome reported increased fruit and vegetable intake and greater lean body mass after the 8 week intervention!
Since college is a time of transition for most students, it offers an opportunity
for sustainable behavioral and lifestyle changes. The central aim of this project
along with the participant population are expected to make a significant impact
on our society’s future.
Identifying metabolic markers of poor health in individuals at risk for metabolic syndrome and related co-morbidities is an innovative and essential component to successful chronic disease prevention. Indeed, the isolation and analysis of a marker will enable healthcare professionals to assess the extent of an individual’s risk for a given disease and subsequently design a personalized nutrition prescription.
Acknowledgements
Fruvedomics is a multidisciplinary pilot project involving West Virginia University researchers from various disciplines, including nutrition, metabolomics, microbiome, cardiovascular, microcirculation, and physiology, along with researchers from the HCRC group. Learn more about HCRC.
This work was supported by a research grant (#2014-67001-21851) from the USDA National Institute of Food and Agriculture, West Virginia Clinical Translational Science Institute (NIH P30 GM103488), and West Virginia University Experimental Station Hatch WVA00627 and WVA00641. WVU Pediatrics; Obesity, Diabetes & Asthma Fund.
Publications & Presentations
* denotes Research Assistant Trainees in the Olfert Research Lab
Famodu OA*, Barr ML*, HoláskováI, Murray PJ, Olfert IM, McFadden JW, Cuff CF, Downes
MT, Colby SE, Coombs D, Buerkle M, Gilleland D,
Olfert MD.Impact of a Nutritional Intervention Focusing on Fruits and
Vegetables on Dietary Intake and Metabolic Parameters in Young Adults.
Personalized Medicine
2018. in review
Clark RL*, Famodu OA*, Holásková I, Infante AM, Murray PJ, Olfert IM, McFadden JW, Downes MT, Chantler PD, Duespohl MW, Cuff CF, Olfert MD. Educational Intervention Alters Diet Composition and Gut Microbiome in Young Adults with Metabolic Syndrome Components. Nutrition Research 2018. in review
Olfert MD, Clark RL*, Famodu OA*, Murray PJ, Olfert IM, Downes MT, McFadden
JW, Cuff CF, Colby SE, Morrell JS. Development of an At-Risk for Metabolic Syndrome
Score.
Topics in Clinical Nutrition
2018. in review
Clark RL*, Famodu OA*, Barr ML*, Hagedorn RL*, Ruseski J, White JA*, Warner C*,
Morrell A*, Murray PJ, Olfert IM, McFadden JW, Downes MT, Colby SE,
Olfert MD. Monetary Cost of the MyPlate Diet in Young Adults: Higher
Expenses Associated with Increased Fruits and Vegetables Consumption.
Nutrients
2018. in review
Famodu OA*,
Olfert MD, Phipps ZC, Cockburn AF, Infante AM, HoláskováI, Downes MT,
Murray PJ, McFadden JW, Olfert IM, Colby SE, Cuff CF. Nutrition Intervention
Impact on the Microbiomes of Young Adults in the FRUVEDomics Pilot Study.
2018. in review
Olfert MD, Famodu OA*, Clark RL, Olfert IM, McFadden JW, Cuff CF, Murray
PJ, Colby SE. FRUVEDomic Study Protocol. (in prep).
_______________________________
Famodu OA*. Designing a Nutrition Intervention to Profile the Human Metabolome, Microbiome & Vascular Health. 3-Minute Research Competition. WVU Mountainlair , Morgantown, WV. April 9, 2015. (Placed 2nd in first round cut $50 award)
Famodu OA*, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Holaskova I, Olfert MD. Designing a Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot. Appalachian Translational Research Network (ATRN). Charleston, WV. October 14-16, 2015. (Travel award $200)
Famodu OA*, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Olfert MD. Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. Mayo Clinic Metabolomics Meeting. Presentation conducted from Rochester, MN, October 25-27, 2015. (Travel award $750)
Famodu OA*, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Cockburn A, Olfert MD. Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study, IDeA Conference (NIH CTSI), Biloxi, MS, 2015. Nov. 11-13, 2015, Biloxi, (Oral Presentation award $500; Travel award $750)
Famodu O, Olfert I, Kesner D, Buerkle M, White J, Barr M, Downs M, Moore C, Murray P, McFadden J, Cuff C, Colby S, Morrell J, Olfert MD. Designing a Nutrition Intervention to Profile Metabolic, Metabolomic, Microbiome and Vascular Health in Young Adults At Risk for Disease: FRUVEDomic Pilot Study. Van Liere Research Symposium Morgantown, WV. February 2015.
Famodu OA*, Olfert IM, Murray PJ, McFadden JW, Cuff CC, Chantler P,
Olfert MD. Designing a Nutrition Intervention to Impact Metabolic, Microbiome
and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study.
Davis College Research and Creativity Expo. Presentation conducted from
Davis College of Agriculture, Natural Resources and Design, Morgantown, WV. April
7,
2015.
Famodu OA*, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Olfert MD. Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study, School of Pharmacy Research Day. Health Sciences Center, Morgantown, WV, June 18, 2015.
E.J. Van Liere & Health Sciences Center Research Day, Morgantown, WV. Feb 26 Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Olfert MD. Designing a Nutrition Intervention to Profile the Human Metabolome, Microbiome & Vascular Health. 2015
3-Minute Thesis Research Competition, Morgantown, WV. April 9. Presentation: Famodu OA. Designing a Nutrition Intervention to Profile the Human Metabolome, Microbiome & Vascular Health. 2015
Hagedorn RL*, White J*, Famodu OA*, Barr M*, Olfert MD . Community Research through Teen Implementation. Undergraduate Research Day at the Capital. Charleston, WV March 4, 2015.
Quispe J*, Famodu OA*, Olfert MD. Impact of Fruits and Vegetables on Cholesterol Levels of at Risk Young Adults. Summer Undergraduate Research Experience Meeting (SURE), Morgantown, WV, July 23, 2015.
School of Pharmacy Research Day, Morgantown, WV. June 18. Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Olfert MD. Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. 2015
Appalachian Translational Research Network (ATRN), Charleston, WV. Oct 14-16. Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Holaskova I, Olfert MD. Designing a Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot. 2015
Mayo Clinic Metabolomics Meeting, Rochester, MN. October 25-27 Presentation: Famodu OA, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Olfert MD. Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. 2015
SE IDeA Conference (NIH CTSI), Biloxi, MS. Nov 11-13 Oral Presentation: Famodu OA, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Cockburn A, Olfert MD. Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study 2015
Experimental Biology (EB), American Society of Nutrition (ASN), San Diego, CA. April 2-6. Abstract: Famodu OA, Cuff CF, Cockburn A, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell JS, Olfert IM, Olfert MD. Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: Fruvedomic Pilot Study. 2016
E.J. Van Liere & Health Sciences Center Research Day, Morgantown, WV. March 3 Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Cockburn AF, Holaskova I, Downes M, Colby SE, Morrell JS, Chantler P, Olfert MD. Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: FRUVEDomics Pilot Study 2016
Famodu OA*, Cuff CF, Cockburn A, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell JS, Olfert IM, Olfert MD. Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: Fruvedomic Pilot Study. Van Liere Research Day, Morgantown, WV March 1, 2016. (1st Place Award for Poster Presentation).
Famodu OA*, Cuff CF, Cockburn A, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell JS, Olfert IM, Olfert MD. Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: Fruvedomic Pilot Study. Experimental Biology (EB), American Society of Nutrition (ASN), San Diego, CA. April 2-6, 2016.
Famodu OA*, Cuff CF, Cockburn A, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell JS, Olfert IM, Olfert MD. Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: Fruvedomic Pilot Study. Experimental Biology (EB), American Society of Nutrition (ASN), San Diego, CA. April 2-6, 2016. (MARC Experimental Biology Scholarship $1850)
Mathews AT, Famodu OA*, Olfert MD, Murray PJ, Cuff CF, Downes MT, Haughey NJ, Morrell JS, Colby SE, Olfert IM, McFadden JW. Fruit and Vegetable Intervention Lowers Circulating Ceramide Levels and Improves Estimated Insulin Sensitivity in Young Adults at Risk of Developing Metabolic Syndrome: A FRUVEDomic Pilot Study . Van Liere Research Day, Morgantown, WV March 1, 2016. (1st Place Award for Oral Presentation).
Mathews AT*, Famodu OA*, Olfert MD , Murray PJ, Cuff CF, Downes MT, Haughey NJ, Morrell JS, Colby SE, Olfert IM, McFadden JW. Fruit and Vegetable Intervention Lowers Circulating Ceramide Levels and Improves Estimated Insulin Sensitivity in Young Adults at Risk of Developing Metabolic Syndrome: A FRUVEDomic Pilot Study. Experimental Biology (EB), American Physiologic Society (APS), San Diego, CA. April 2-6, 2016.
Moore CM*, Fournier SF, DeVallance E, Lemaster, K, Olfert MD, Olfert. IM, Chantler PD. Does 8-weeks of Increased Fruit and Vegetable Dietary Intervention Improve Arterial Hemodynamics: FRUVEDomic Pilot Study. Van Liere Research Symposium Morgantown, WV. March 2016.
Olfert MD, Famodu OA, Cuff C, Cockburn A, Olfert IM,McFadden JW, Downes ME, Murray PJ, Holaskova I, Barr ML, Colby SE, Morrell J.Nutrition Intervention to Profile Microbiome and Behaviors in Young Adults atRisk for Metabolic Syndrome: FRUVEDomic Pilot Study. JNEB Volume 48, Issue 7, S145, 2016
Olfert MD, Famodu OA*, Barr ML, Murray PJ, McFadden JW, Cuff CF, Colby SE, Morrell JS, Olfert IM. Team Science Approach Helps to Identify how Fruit and Vegetable Consumption Improves Behavioral Health in Free-Living Young Adults: A Fruvedomic Pilot Study. NISBRE.Washington DC, July 26-28, 2016
Olfert IM, MAthews AT, Famodu OA*, Olfert MD, Cuff CF, Murray PJ, Haughey NJ, Colbey SE, McDadden JW. Team Science Approach Helps to Identify how Fruit and Vegetable Consumption Improves Metabolic Health: A Fruvedomic Pilot Study. NISBRE. Washington DC, July 26-28, 2016.
Society for Nutrition Education and Behavior (SNEB), San Diego, CA. July 30 Abstract: Famodu OA, Cuff C, Cockburn A, Olfert IM, McFadden JW, Downes ME, Murray PJ, Holaskova I, Barr ML, Colby SE, Morrell J, Olfert MD. Nutrition Intervention to Profile Microbiome and Behaviors in Young Adults at Risk for Metabolic Syndrome: FRUVEDomic Pilot Study. SNEB Annual Meeting. 2016
Famodu OA, Cuff C, Cockburn A, Olfert IM, McFadden JW, Downes ME, Murray PJ, Holaskova I,Barr ML, Colby SE, Morrell J, Olfert MD. Nutrition Intervention to Profile Microbiome and Behaviors in Young Adults at Risk for Metabolic Syndrome: FRUVEDomic Pilot Study. SNEB Annual Meeting, San Diego, CA. July 30, 2016.
Clark RL, Famodu OA, Barr ML, Murray PJ, Olfert IM, Downes MT, Cuff CC, McFadden JW, Chantler PD, Colby SE. Fruit and Vegetable Diet Intervention in Young Adults with Metabolic Syndrome: Fruvedomics Pilot Study. Van Liere Research Symposium. Morgantown, WV. February 2017.
Hagedorn RL*, Barr ML*, Famodu OA*, Clark RL*, Morris AM*, Wattick RA*, Waanders T, Olfert MD. Behaviour Change Counseling: Training Graduate Dietetic Students in Shortened Motivational Interviewing. The FASEB Journal 31 (1 Supplement), 975.5-975.5. Chicago, Illinois. April 22-26. 2017.
Clark RL, Famodu OA, Barr ML, Murray PJ, Olfert IM, Downes MT, Cuff CC, McFadden JW, Chantler PD, Colby SE: Fruit and Vegetable Diet Intervention in Young Adults with Metabolic Syndrome: Fruvedomics Pilot Study. The FASEB Journal 2017, 31(1 Supplement):970.979-970.979. Experimental Biology. Chicago, IL. April 2017.
Clark RL*, Famodu OA*, Barr ML*, Murray PJ, Olfert MI, Downes MT, Cuff CC, McFadden JW, Chantler PD, Colby SE, Olfert MD. Fruit and Vegetable Diet Intervention in Young Adults with Metabolic Syndrome: Fruvedomics Pilot Study. The FASEB Journal 31 (1 Supplement), 970.9-970.9 . Chicago, Illinois. April 22-26. 2017.
Famodu OA*, Infante AM, Cuff CC, Holaskova I, Olfert IM, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell J, Olfert MD. Microbiome Impact from a Nutrition Intervention in Young Adults: FRUVEDomics Pilot Study. The FASEB Journal 31 (1 Supplement), 965.41-965.41. Chicago, Illinois. April 22-26. 2017.