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FRUVEDomics

Fruvedomics is a WVCTSI-funded pilot study focused on the identification of biomarkers for poor metabolic, cardiovascular, and microbiome health, as well as their sensitivity to nutritional intervention in young adults.

Introduction

Chronic diseases are a major public health concern nationwide, but particularly in the health disparate regions often seen in rural states, such as West Virginia. It is becoming increasingly critical that we identify the distinct role of nutrition in the etiology of chronic diseases and understand how specific diets influence the metabolome. Furthermore, isolating specific biomarkers and determining their effects on the microbiome will be necessary to the development of individualized therapeutic diet plans that effectively prevent chronic diseases both at the individual level and on a much larger community scale.

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Aims

Fruvedomics uses a translational approach to a free-living, nutrition intervention based on the 2010 Dietary Guidelines for Americans. This study aims to investigate dietary impact on the metabolome, microbiome, and cardiovascular system; identify novel metabolic phenotypes; and assess behavior change outcomes in college-aged students at-risk of metabolic syndrome.

blood pressure measurement

Design

Fruvedomics is a translational clinical research trial. A total of 36 subjects, most of whom screened as “at risk” for metabolic syndrome, participated in an 8-week nutritional intervention study. Subjects were randomized into one of three groups:
1) “Fruved” diet with 50% fiber, fruit, and vegetable
2) “Fruved” and low simple-carbohydrate diet (Fruved+LowCHO)
3) “Fruved” and low-fat diet (Fruved+LowFat).

As part of our metabolomics approach, venous blood samples were collected for amino acid, carbohydrate, fatty acid, and sphingolipid metabolism. Collections occured pre- and post-nutritional intervention, and at weeks three and five of the program, for a total of four repeated blood samples for metabolomic assessment. Additionally, anthropometrics, body composition, stool samples, and arterial stiffness measurements were recorded pre- and post-intervention. Finally, participants answered a ~300-question lifestyle behaviors survey pre- and post-intervention.

Diet were measured both subjectively (food pictures/diet logs) and objectively (nutritionist professional/food receipts) on a weekly basis throughout the eight-week intervention period. Each week the subject was incentivized to have their logs, receipts, and pictures reviewed by a Registered Dietitian Nutritionist in order to facilitate counseling and ensure compliance with the diet.
Check out some post intervention results.

Micriobiologist at work

Outcomes, Impact, & Future Work

Preliminary findings of "at-risk" students show improved metabolic health after a diet intervention of increased fruits and vegetables. Changes in the metabolome and microbiome were found, with results indicating less inflammation, which is associated with lower disease risk. Individuals with metabolic syndrome reported increased fruit and vegetable intake and greater lean body mass after the 8 week intervention!

Since college is a time of transition for most students, it offers an opportunity for sustainable behavioral and lifestyle changes. The central aim of this project along with the participant population are expected to make a significant impact on our society’s future.

Identifying metabolic markers of poor health in individuals at risk for metabolic syndrome and related co-morbidities is an innovative and essential component to successful chronic disease prevention. Indeed, the isolation and analysis of a marker will enable healthcare professionals to assess the extent of an individual’s risk for a given disease and subsequently design a personalized nutrition prescription.

Acknowledgements

Fruvedomics is a multidisciplinary pilot project involving West Virginia University researchers from various disciplines, including nutrition, metabolomics, microbiome, cardiovascular, microcirculation, and physiology, along with researchers from the HCRC group. Learn more about HCRC.

This work was supported by a research grant (#2014-67001-21851) from the USDA National Institute of Food and Agriculture, West Virginia Clinical Translational Science Institute (NIH P30 GM103488), and West Virginia University Experimental Station Hatch WVA00627 and WVA00641. WVU Pediatrics; Obesity, Diabetes & Asthma Fund.

Publications, Posters, & Presentations

FamoduOA, Olfert IM, Barr ML, White JA, Warner C, Hagedorn RL, Starrett P, Dehlin M, Hass A, Rubino J, Mathews A,Aromeh L, McFadden JW, Murray PJ, Olfert MD. Nutrition Intervention to ImpactMetabolic and Vascular Health.  Clinical Translational Science InstituteHealth Disparity and STEM Meeting. Presentation conducted from DavisCollege of Agriculture, Natural Resources and Design, Morgantown, WV. Nov. 19,2014.

Famodu O, Olfert I, Kesner D, Buerkle M, White J, Barr M, Downs M, Moore C, Murray P, McFadden J, Cuff C, Colby S, Morrell J, Olfert M. Designing a Nutrition Intervention to Profile Metabolic, Metabolomic, Microbiome and Vascular Health in Young Adults At Risk for Disease: FRUVEDomic Pilot Study. Van Liere Research Symposium Morgantown, WV. February 2015.

E.J. Van Liere & Health Sciences Center Research Day, Morgantown, WV. Feb 26 Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Olfert MD.  Designing a Nutrition Intervention to Profile the Human Metabolome, Microbiome & Vascular Health. 2015 

Davis College Research and Creativity Expo,Morgantown, WV. April 7. Presentation: Famodu OA, Olfert IM, Murray PJ,McFadden JW, Cuff CC, Chantler P, Olfert MD.  Designing a Nutrition Intervention to Impact Metabolic, Microbiome andVascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. 2015 

3-Minute Thesis Research Competition, Morgantown, WV. April 9. Presentation: Famodu OA. Designing a Nutrition Intervention to Profile the Human Metabolome, Microbiome & Vascular Health.2015 

School of Pharmacy Research Day, Morgantown, WV. June 18. Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Olfert MD.  Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. 2015 

Society for Nutrition Education and Behavior(SNEB),  July 25-29 Presentation: Famodu OA, Olfert IM, Kesner D, BuerkleM, White JA, Barr ML, Woehrer K, Downes MT, Moore C, Murray PJ, McFadden JW,Cuff CF, Colby SE, Morrell JS, Olfert MD.  Designing a Nutrition Intervention to Profile Metabolic, Metabolomic,Microbiome and Vascular Health in Young Adults At Risk for Disease: FRUVEDomicPilot Study.    J Nutr Educ Behav 2015;47(4) S65.         2015 

Appalachian Translational Research Network (ATRN), Charleston, WV. Oct 14-16. Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Chantler P, Holaskova I, Olfert MD. Designing a Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot. 2015 

Mayo Clinic Metabolomics Meeting, Rochester, MN. October 25-27 Presentation: Famodu OA, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Olfert MD.  Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study. 2015 

SE IDeA Conference (NIH CTSI),  Biloxi, MS. Nov 11-13 Oral Presentation: Famodu OA, Olfert IM, Murray PJ, Downes MT, McFadden JW, Cuff CF, Cockburn A, Olfert MD.  Nutrition Intervention to Profile Metabolic, Microbiome and Vascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study 2015   

Famodu O, Olfert I, Kesner D, Buerkle M, White J, Barr M, Downs M, Moore C, Murray P, McFadden J, Cuff C, Colby S, Morrell J, Olfert M. Designing a Nutrition Intervention to Profile Metabolic, Metabolomic, Microbiome and Vascular Health in Young Adults At Risk for Disease: FRUVEDomic Pilot Study. J Nutr Educ Behav 2015; 47(4) S65. DOI:http://dx.doi.org/10.1016/j.jneb.2015.04.171

Experimental Biology (EB), American Society of Nutrition (ASN), San Diego, CA. April 2-6. Abstract: Famodu OA, Cuff CF, Cockburn A, Downes MT, Murray PJ, McFadden JW, Colby SE, Morrell JS, Olfert IM, Olfert MD.  Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: Fruvedomic Pilot Study. 2016 

E.J. Van Liere & Health Sciences Center Research Day, Morgantown, WV. March 3 Presentation: Famodu OA, Olfert IM, Murray PJ, McFadden JW, Cuff CF, Cockburn AF, Holaskova I, Downes M, Colby SE, Morrell JS, Chantler P, Olfert MD.  Impact of Free-Living Nutrition Intervention on Microbiome in College Students at Risk for Disease: FRUVEDomics Pilot Study 2016 

Society for Nutrition Education and Behavior (SNEB), San Diego, CA. July 30 Abstract: Famodu OA, Cuff C, Cockburn A, Olfert IM, McFadden JW, Downes ME, Murray PJ, Holaskova I, Barr ML, Colby SE, Morrell J, Olfert MD. Nutrition Intervention to Profile Microbiome and Behaviors in Young Adults at Risk for Metabolic Syndrome: FRUVEDomic Pilot Study.  SNEB Annual Meeting. 2016 

WVU’s 42nd Hal Wanger Family MedicineConference, Morgantown, WV. August 15 Abstract: Famodu OA, Olfert MD, HolaskvaI, Barr ML, Zhou W, Morrell JS, Colby SE.  Designing a Nutrition Intervention to Impact Metabolic, Microbiome andVascular Health in Young Adults at Risk for Disease: FRUVEDomic Pilot Study.  2016 

West Virginia Rural Health Conference, Logan,WV. October 19-21 Abstract: Famodu OA, Olfert MD, Holaskva I, Barr ML, Zhou W,Morrell JS, Colby SE.  Designinga Nutrition Intervention to Impact Metabolic, Microbiome and Vascular Health inYoung Adults at Risk for Disease: FRUVEDomic Pilot Study.  2016

Famodu OA, Cuff C, Cockburn A, Olfert IM, McFadden JW, Downes ME, Murray PJ, Holaskova I,Barr ML, Colby SE, Morrell J, Olfert MD. Nutrition Intervention to Profile Microbiome and Behaviors in Young Adults at Risk for Metabolic Syndrome: FRUVEDomic Pilot Study. SNEB Annual Meeting, San Diego, CA. July 30, 2016.

Clark RL, Famodu OA, Barr ML, Murray PJ, Olfert IM, Downes MT, Cuff CC, McFadden JW, Chantler PD, Colby SE. Fruit and Vegetable Diet Intervention in Young Adults with Metabolic Syndrome: Fruvedomics Pilot Study. Van Liere Research Symposium. Morgantown, WV. February 2017.  

Hagedorn RL, Barr ML, Famodu OA, Morris AM, Clark RL, Olfert MD: Food Insecurity Among College Students at West Virginia University and Self-Reported Health Status. The FASEB Journal 2017, 31(1 Supplement):791.732-791.732. Experimental Biology. Chicago, IL. April 2017. 

Hagedorn RL, Barr ML, BS, Famodu OA, Clark RL, Morris AM, Wattick RA, Waanders TR, Olfert MD. Behavior Change Counseling: Training Graduate Dietetic Students in Shortened Motivational Interviewing.  Experimental Biology. Chicago, IL. April 2017.

Clark RL, Famodu OA, Barr ML, Murray PJ, Olfert IM, Downes MT, Cuff CC, McFadden JW, Chantler PD, Colby SE: Fruit and Vegetable Diet Intervention in Young Adults with Metabolic Syndrome: Fruvedomics Pilot Study. The FASEB Journal 2017, 31(1 Supplement):970.979-970.979. Experimental Biology. Chicago, IL. April 2017.  

 Clark RL, Famodu OA, Morrill A, Warner C, Ruseski J, Murray PJ, Colby S, Olfert MD: Cost Increase to Follow USDA MyPlate Diet in College-Aged Students. Journal of Nutrition Education and Behavior 2017, 49(7):S31. SNEB Annual Meeting.Washington DC. July 2017. doi:10.1016/j.jneb.2017.05.307